Syk Knockout cell line （BV2）

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location： Home > Products > KO Cell Lines > Syk Knockout cell line （BV2）

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Syk Knockout cell line （BV2） Order

Syk Knockout cell line （BV2）
Catalog#
YKO-M202
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Syk Knockout cell line （BV2）
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Syk Knockout cell line (BV2)
Morphology
Epithelial-like,?adherent
Passage ratio
1:3~1:5
Species
Mouse
Gene Name
Syk
Gene ID
20963
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript Syk-203)
Validation: PCR+Sanger Sequencing
Syk Knockout cell line （BV2） Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Syk gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BV2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Syk gene knockout Syk Knockout cell line (BV2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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